Find genomic
region of interest

Find primers in
region of interest
using HD FISH
primers database

Synthesize
primers in
96-well plate

Perform real-time PCR;
select amplicons with
similar amplification
kinetics

Label
amplicons
with
Universal
Linkage
Systemâ„¢

Hybridize probe(s)
to visualize your
DNA region of
interest in cells